Journal: Laboratory Animal Research

Article Title: Comparison of intrinsic exercise capacity and response to acute exercise in ICR (Institute of Cancer Research) mice derived from three different lineages

doi: 10.1186/s42826-021-00094-0

Figure Lengend Snippet: Comparative analysis of the cardiac mitochondrial oxidative phosphorylation (OXPHOS) complex, mitochondrial function, and uncoupling protein (UCP) 3 expression levels among the ICR lineages. A Representative western blots showing the protein levels of the OXPHOS complex. B The densitometric analysis of blots normalized for COX-IV in the cardiac mitochondrial fraction of different ICR lineages. C Oxygen consumption rate (OCR) in the permeabilized cardiac muscles of different ICR lineages was recorded using the Oroboros O2K instrument. D The respiratory control ratio (RCR) was calculated as the ratio of oxygen consumption at state 3 (complex 1 and complex 1 + 2) to that at state 2. E Representative western blots showing the protein levels of UCP3 in the soleus, extensor digitorum longus (EDL), and cardiac muscle. F The densitometric analysis of blots normalized for the density of Ponceau staining in different muscles. The values are presented as mean ± SEM; each group consisted of 5–6 mice. * P < .05, ** P < .01, and *** P < .001

Article Snippet: C Oxygen consumption rate (OCR) in the permeabilized cardiac muscles of different ICR lineages was recorded using the Oroboros O2K instrument.

Techniques: Phospho-proteomics, Expressing, Western Blot, Muscles, Control, Staining

Journal: Laboratory Animal Research

Article Title: Comparison of intrinsic exercise capacity and response to acute exercise in ICR (Institute of Cancer Research) mice derived from three different lineages

doi: 10.1186/s42826-021-00094-0

Figure Lengend Snippet: Comparative analysis of the metabolic phenotypes of different ICR lineages during a 24-h period at resting metabolic rate (RMR). A Schematics of the experimental design and protocol. B Changes in oxygen uptake and total oxygen uptake during different phases (total, inactive, and active). C Changes in CO 2 production and total CO 2 production during different phases. D Changes in the respiratory exchange ratio (RER) and average RER during different phases. The shaded part of the line graph represents the dark period of the day cycle. The values are presented as mean ± SEM; each group consisted of four mice

Article Snippet: C Oxygen consumption rate (OCR) in the permeabilized cardiac muscles of different ICR lineages was recorded using the Oroboros O2K instrument.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: A CRISPR-edited isoform of the AMPK kinase LKB1 improves the response to cisplatin in A549 lung cancer cells

doi: 10.1016/j.jbc.2025.108308

Figure Lengend Snippet: Super LKB1 enhances the oxidative metabolism in A549-edited cells. A , Western blotting of HK2, LDHA, and GAPDH in cWT, c2SL+, and c3SL + cells; ( B ) normalized levels of proteins by GAPDH (a.u); ( C ) Western blotting of OXPHOS and GAPDH in cWT, c2SL+, and c3SL + cells. D , normalized levels of proteins by GAPDH (a.u); ( E ) oxygen consumption rate (OCR) by OROBOROS in cWT, c2SL+, and c3SL + cells; ( F ) comparison levels of O 2 in the cell lines in the basal condition and after treatments with oligomycin and FCCP. The data are presented as mean ± SD. Statistical analysis was performed by ANOVA followed by Dunnet’s test ∗ p < 0.05, ∗∗ p < 0.01. These data are representative of two independent experiments.

Article Snippet: D , normalized levels of proteins by GAPDH (a.u); ( E ) oxygen consumption rate (OCR) by OROBOROS in cWT, c2SL+, and c3SL + cells; ( F ) comparison levels of O 2 in the cell lines in the basal condition and after treatments with oligomycin and FCCP.

Techniques: Western Blot, Comparison

Journal: PLoS ONE

Article Title: Protective Effect of Boerhaavia diffusa L. against Mitochondrial Dysfunction in Angiotensin II Induced Hypertrophy in H9c2 Cardiomyoblast Cells

doi: 10.1371/journal.pone.0096220

Figure Lengend Snippet: Reduction in fluorescence indicates lower oxygen consumption rate in the cells. Results expressed as mean ± SD; n = 6. * indicates significant difference from group I and # indicates significant difference from group III ( P ≤0.05). Group I- Control cells; Group II-BDE (75µg/ml) alone treated cells; Group III-Ang II (100 nm) treated cells; Group IV-Ang II+BDE treated cells.

Article Snippet: Oxygen consumption rate in control and treated cells were assayed using Cayman’s cell based oxygen consumption rate assay kit using antimycin A as standard inhibitor (Cayman Chemicals, Ann Arbor, USA)

Techniques: Fluorescence, Control